Corn Export Cargo Quality Report 2012/2013

E. Mycotoxin Testing

Official aflatoxin results are provided by FGIS for the Export Cargo Report 2012/13. For the aflatoxin testing, a sample of at least 10 pounds of shelled corn was used according to FGIS official procedures. The 10-pound sample was ground using a FGIS-approved grinder. Following the grinding stage, two 500-gram ground portions are removed from the 10-pound comminuted sample using a riffle divider. From one of the 500-gram ground portions, a 50-gram test portion is randomly selected for testing. After adding the proper extraction solvent to the 50-gram test portion, aflatoxin is quantified. The following FGISapproved quantitative test kits may have been used: VICAM AflaTest™, Beacon Analytical Plate Kit, Romer Labs filuoroQuant Afla IAC, Envirologix QuickTox™ for QuickScan Aflatoxin, Neogen Reveal Q+ for Aflatoxin or Veratox® Aflatoxin Quantitative Test, Charm Sciences ROSA® FAST or WET™ Aflatoxin Quantitative Test, or R-Biopharm RIDASCREEN® FAST Aflatoxin SC test.

For the DON testing, the FGIS-approved Envirologix QuickToxâ„¢/QuickScantest method was used. A 1350- gram sample of shelled corn (obtained by dividing the original sample) was ground to a particle size which would pass through a number 20 wire mesh sieve and divided down to a 50-gram test portion using a Romer Model 2A sampling mill. The 50-gram test portion was then processed as the FGIS DON (Vomitoxin) Handbook requires. DON was extracted with 250 ml of distilled water, and the extract was tested using the Envirologix AQ 204 BG test kits. The DON was quantified using the QuickScanReader.